Demand the Bigger Picture
Long String STAR V Demand the Bigger Picture
The Long String STAR V
EXPANDING THE FIELD OF VIEW THROUGH INNOVATION
In this ever-evolving field, new innovations continue to shed light on this uncharted territory and drive new discoveries. Bionano Genomics has developed Optical Genome Mapping (OGM), a technique that transforms the view of the genome, by detecting structural variants in an unbiased, genome-wide fashion. In contrast to conventional sequencing techniques, OGM leverages the extraction of Ultra High Molecular Weight (UHMW) DNA. Thanks to Hamilton’s focus and long-standing expertise in automating genomic workflows, we are now stepping into the most advanced applica- tion areas. With the Long String STAR V for UHMW DNA extraction, we are now proudly presenting the world’s first automation solution for this process. In conjunction with OGM, you will be able to see what has been hidden for so long. Take the next step towards answering open questions in genomic analysis, while working efficiently and at scale.
PIONEERING TOWARDS BETTER RESOLUTION OF STRUCTURAL VARIATION
Over the past few decades, the understanding of biological principles and processes has evolved tremendously. An invaluable contribution towards this gain in knowledge is attributable to advances in genomic research and technologies. Next-Generation Sequencing (NGS) approaches, as well as other techniques, have had a fundamen- tal impact on life sciences and many applied fields within our society. But the journey is far from over. Although well established, current NGS methods are unable to accurately resolve certain regions of the genome. This makes the detection of structural variants particularly challenging. Different cyto- genetic techniques have their own limitations as well. Even in concert, these methods cannot pro- vide a complete and high-resolution picture of the genome. This inability represents one of the last frontiers in genomics.
Conventional DNA Extraction Short-Read Sequencing Data 150 bp / Molecule
UHMW DNA Extraction * Optical Genome Mapping Data ≥ 230,000 bp / Molecule
Throughout this brochure, protected product names may be used without being specifically marked as such.
*The DNA isolated from the Hamilton Long String STAR V contains Mbp length molecules which overall exceed the OGM quality threshold of 230 kbp post extraction, labeling, linearization, and imaging.
Together is Better
As experts in precision automated liquid handling, Hamilton has partnered with Bionano Genomics to automate the UHMW DNA extraction process. Together, we empower the scientific community by accelerating its ability to process a high number of samples in a reliable and reproducible manner. Our aim is to always provide the best possible solution, to elevate our customers.
Extract Longer DNA Molecules Easier Than Ever Before
Although structural variants make a significant contribu- tion to human disease, such aberrations are frequently missed by NGS technology. The analysis of UHMW DNA by OGM provides a more comprehensive way of detecting such aberrations in the range of 500 bp up to megabase pairs in length. Structural variation discov- ery with OGM outperforms what can be achieved by sequencing-based workflows.
The manual preparation of UHMW DNA samples can be challenging, due to sample viscosity and sensitivity to shearing. Manual protocols require users to be highly trained to achieve optimal results with regard to both quantity and quality. These factors also limit the number of samples that can be reasonably processed at a single time. Long String STAR V optimizes the laboratory workflow for purification of ultra-long DNA molecules, increasing confidence about your sample quantity and quality. It drives consistency, as extracts are always prepared with identical parameters, ensuring great yields and excellent quality. Adopt a full walk-away workflow for UHMW DNA extraction with increased sample throughput to take your sample preparation process to the next level. Replace manual preparations with consistent, auto- mated protocols Consistently obtain UHMW DNA molecules Standardize and trace your sample workflow Reduce hands-on time for improved lab efficiency Process up to 12 samples per run and 24 samples in a single workday
Up to 99% structural variant detection
10,000X resolution compared to karyotype Routinely detect mosaic variants down to as little as 5% variant allele fraction To capitalize on these benefits, UHMW DNA (typically between 250 kbp up to > 1 Mpb in length) must be isolated from samples of interest. Instead of magnetic beads or silica spin columns, the procedure uses magnetic disks, which are covered by a nanostructured silica surface. This enables a high DNA binding capacity and helps to protect the bound DNA from shearing forces.
The Tool for Novel Discoveries
IN FULL CONTROL OF EVERY STEP FOR MORE CONFIDENCE
Hamilton MagRods are specifically designed for optimal magnetic disk handling. MagRods can be operated with 5 mL CO-RE pipetting channels and therefore offer a broad range of flexibility. Handle magnetic disks anywhere on-deck Adjustable settings for both speed and range of movement Tunable to cater to sample type or workflow require- ments Freedom to optimize even single steps
The Long String STAR V is an Assay Ready Workstation with a standardized hardware configuration for automated magnetic disk handling. The product is suitable for Research Use Only (RUO). It is based on our latest and greatest Microlab STAR V platform and features state-of-the-art CO-RE pipetting tech- nology, as well as the Hamilton Quad CO-RE Gripper for plate transport steps. Our customers benefit from a turnkey solution that minimizes implementation times. Qualified Methods are being developed, together with our partners, and distributed along with the instrument. More than that, we value the strong support for our clients which serves well beyond initial installation.
EVERYTHING YOU NEED, WHENEVER YOU NEED IT
Hamilton MagRod Sleeves
Hamilton MagRod Sleeves are manufactured with the highest precision and are shaped for the best possible magnetic disk positioning. The perfect fit protects the MagRods from surrounding liquids and reagents.
For additional information about the Long String STAR V, including an explanatory animation video and an Application Note with biological testing results, please access our webpage via the QR Code below.
Hamilton Elution Strips
Hamilton Elution Strips are used to separate the magnetic disks from the final UHMW DNA sample by centrifugation. The disk remains in the strip, while the eluate is drawn into a microtiter plate.
All necessary consumables are available directly from Hamilton and can be purchased separately. Free of human DNA, DNase, and RNase Sterile, in compliance with ISO 11135 Packaging size matches throughput needs
Click, Click, Go
SETTING A SOLID FOUNDATION FOR OPTICAL GENOME MAPPING
We have designed the operator interface and programming styles with user friendliness in mind for faster familiarization. Intuitive editors offer simple ways to set up and run your method, with predefined parameters optimized by a team of Scientists from both Bionano Genomics and Hamilton. At the same time, you have full control over all method aspects.
UHMW DNA EXTRACTION
Lysis and Digestion
DNA Binding to Disks
Disk Transfer to Elution Plate
Final Elution by Centrifugation
More time to focus on steps ahead
Easily viewable color-coded lights indicate progress Customizable worklist templates for quick editing Run simulations for method optimization Loading dialogs specify required consumables and reagents Helper tools for new workflows and separate test methods Selected highlights include:
DIRECT LABEL AND STAIN (DLS) PROCESS
The established protocol is divided into simple lyse, bind, wash, and elution procedures that can be performed using kits from Bionano Genomics. Herein, genomic DNA is bound to, purified on, and released from a paramag netic disk. Frozen cell pellets are manually resuspended and provided to the system (automated resuspension of cell pellets supported by the method). Subsequent steps are per- formed entirely on the Long String STAR V instrument. For final elution, the disks are transferred to strips pre-filled by the instrument with elution buffer, standing on top of a microtiter plate. Via a short off-deck centrifugation step, the final UHMW DNA samples are collected in the plate wells. The extracts obtained are suitable for the Bionano Direct Label and Stain (DLS) process, that enzymatically applies sequence-specific label patterns, meant for data collection on the Saphyr ® System.
DATA COLLECTION ON SAPHYR SYSTEM
Long String STAR V Process
Optical Genome Mapping Process
MAPPING AND STRUCTURAL VARIANT ANNOTATION
Processes downstream of UHMW DNA extraction are not covered on the Long String STAR V. For products and services specific to DLS processing, data collection and analysis, please contact Bionano Genomics directly.
THE BASIS OF EVERY WORKFLOW
15 – 35°C
15% – 85% with no condensation
We’re proud of the fact that we produce our own tips at various locations throughout Switzerland and the US. This is the only way we can meet our own high-quality standards; and not only ours, but also the most important ISO standards*.
Height with closed door
up to 2000 m above sea level
Height with open door
Electrical Power Requirements
110 – 230V AC 50/60Hz +-5%
approx. 250 kg
1000 µL Pipetting Channels Technologies
1000 µL Pipetting Channel Specifications
Precision CV (%)
Our goal is to provide the best possible components for our systems with our tips, because they’re the essence of every workflow. Nothing works without the appropriate consumables. That's why we offer an entire range of high-quality consumables that provide more security, higher efficiency, and thus more convenience. We also develop and produce smart consumables, which enable innovative solutions in new applications and research fi elds.
CO-RE ® Technology
capacitive Liquid Level Detection (cLLD)
Tip Type Detection
Long String STAR V
50 µL Filtered Tips
300 µL Filtered Tips
1000 µL Filtered Tips
Hamilton consumables are produced in an ISO Class 8 Clean Room, based on ISO 14644 standards, as well as ISO 9001 and ISO 13485. Hamilton goes beyond relying on just statistical analyses of quality to assure the highest product quality (e.g. each CO-RE tip is checked for concentricity of its orifice and the absence of flash from the molding process).
Long String STAR V Bench Swing Arm for Monitor (only available in combination with Bench)
Deep Well Plates (2.2 mL) PP
20 mL Reagent Reservoirs
Microplate 96/U, Wells Clear, RecoverMax
2.0 mL Screw Cap Microtubes
*For ordering details, please get in touch with your local Hamilton sales representative.
Long String Genomics
OPTICAL GENOME MAPPING - THE FUTURE IS NOW
MOVING FORWARD BY CLOSING GAPS
Use UHMW DNA to perform OGM for unbiased, genome-wide detection of structural variants across a broad range of research applications:
An alternative to traditional cytogenetic workflows to detect more chromosomal aberrations in a sin- gle assay OGM detects the structural variants that are missed by sequencing with higher resolution than traditional cytogenetic methods. Reset the benchmark for detecting chromosomal aberrations.
Using the Long String STAR V in your laboratory will provide you with the opportunity to extract UHMW DNA easier than ever before with sample throughput surpassing manual capabilities. Get the power to bring your workflow performance to the next level. Human handling errors are a thing of the past, and the user-to-user variation seen with manual protocols is replaced by automated consistency and reproducibility. The Long String STAR V from Hamilton and OGM solutions from Bionano Genomics add another layer of discovery to your genetic experiments. With the ability to reliably extract ultra-long DNA molecules paired with the Bionano Saphyr system, all classes of structural variants are revealed and short-read sequencing blindspots can be unveiled. Together, Long String STAR V and OGM deliver a view that empowers you to overcome challenges and discover more.
Constitutional genetic disorders
Cell bioprocessing quality control Gene discovery and therapy Reference genome assembly
Complement data across the variant continuum by combining OGM with NGS-based methods
By combining OGM and NGS, you can characterize both structural and single nucleotide variants, providing a comprehensive analysis of genomic variation that can impact human health. Reveal more that matters for powerful genetic discovery.
You have just turned from page 3 to page 12 and the OGM Data / molecule has covered all pages, while conventional NGS Data has taken only a very small space on page 3.
For more information about potential applications for Optical Genome Mapping with UHMW DNA, visit bionanogenomics.com.
* The DNA isolated from the Hamilton Long String STAR V contains Mbp length molecules which overall exceed the OGM quality threshold of 230 kbp post-extraction, labeling, linearization, and imaging.
Yes, that long!
UHMW DNA Extraction * Optical Genome Mapping Data ≥ 230,000 bp / Molecule
Automating your Imagination
We drive innovation to improve people's lives
We develop and produce state-of-the-art Liquid Handling Systems and laboratory automation technologies. Devote yourself to new ideas, trans- forming your ideas into reality. The entire product portfolio is characterized by maximum reliability, maximum efficiency, flexibility and user-friendliness. From production to service and support, the focus is always on your individual needs.
Hamilton has stood for Life Sciences and Medical Technology since 1966. A visionary idea developed into a strong global presence. Our spirit has always remained the same: To research, develop and produce with passion, courage and curios- ity. The desire to discover new technological solutions, innovations, and benefit humanity is our mission. We strive to set our goals high and continue to meet the measure of excellence.
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Denmark, Norway, Sweden, Finland +46 8410 27 373 France +33 184 008 420 Germany, Austria, Switzerland +49 89 248 804 804
Benelux +31 40 209 178 0 Italy +39 039 930 06 06 Japan +81 3 6435 6850 Spain, Portugal +34 930 186 262
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© 2022 Hamilton Company. All rights reserved. All trademarks are owned and/or registered by Hamilton Company in the U.S. and/or other countries. Lit. No. B-2205-02— 02/2023 Bionano Genomics and Saphyr are trademarks of Bionano Genomics, Inc.Page 1 Page 2-3 Page 4-5 Page 6-7 Page 8-9 Page 10-11 Page 12-13 Page 14-15 Page 16
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