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SETTING A SOLID FOUNDATION FOR OPTICAL GENOME MAPPING
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SAMPLE PREPARATION
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UHMW DNA EXTRACTION
Lysis and Digestion
DNA Binding to Disks
Disk Transfer to Elution Plate
Final Elution by Centrifugation
Disk Washing
We have designed the operator interface and programming styles with user friendliness in mind for faster familiarization. Intuitive editors offer simple ways to set up and run your method, with predefined parameters optimized by a team of Scientists from both Bionano and Hamilton. At the same time, you have full control over all method aspects.
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DIRECT LABEL AND STAIN (DLS)
DLE-1 Labeling
Proteinase K Digestion
DL-Green Clean-up
Homo- genization
DNA Staining
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Easily viewable color-coded lights indicate progress Customizable worklist templates for quick editing Run simulations for method optimization Loading dialogs specify required consumables and reagents Helper tools for new workflows and separate test methods Selected highlights include:
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More time to focus on steps ahead
Off-Deck Process
DATA COLLECTION ON SAPHYR SYSTEM
The established protocol is divided into simple lyse, bind, wash, and elution procedures that can be performed using kits from Bionano. Herein, genomic DNA is bound to, purified on, and released from a paramagnetic disk. Frozen cell pellets are manually resuspended and provided to the system (automated resuspension of cell pellets supported by the method). Subsequent steps are per- formed entirely on the Long String STAR V instrument. For final elution, the disks are transferred to strips pre-filled by the instrument with elution buffer, standing on top of a microtiter plate. Via a short off-deck centrifugation step, the final UHMW DNA samples are collected in the plate wells. After manual quality control and dilution, UHMW DNA samples can be provided back to the system for a fully automated labeling process. Only a final off-deck incuba- tion step is needed before samples can be loaded for data collection on the Saphyr ® and Stratys ® Systems.
Long String STAR V Process
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Optical Genome Mapping Process
ASSEMBLY, CROSS-
MAPPING AND STRUCTURAL VARIANT ANNOTATION
For products and services specific to data collection and analysis, please contact Bionano directly
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