Demand the Bigger Picture
Long String STAR V Demand the Bigger Picture
The Long String STAR V
EXPANDING THE FIELD OF VIEW THROUGH INNOVATION
In this ever-evolving field, new innovations continue to shed light on this uncharted territory and drive new discoveries. Bionano has developed Optical Genome Mapping (OGM), a technique that trans- forms the view of the genome, by detecting struc- tural variants in an unbiased, genome-wide fashion. In contrast to conventional sequencing techniques, OGM leverages the extraction and labeling of Ultra High Molecular Weight (UHMW) DNA. Thanks to Hamilton’s focus and long-standing expertise in automating genomic workflows, we are now stepping into the most advanced applica- tion areas. With the Long String STAR V for UHMW DNA extraction and labeling, we are now proudly presenting the world’s first automation solution for these processes. In conjunction with OGM, you will be able to see what has been hidden for so long. Take the next step towards answering open questions in genomic analysis, while working efficiently and at scale.
PIONEERING TOWARDS BETTER RESOLUTION OF STRUCTURAL VARIATION
Over the past few decades, the understanding of biological principles and processes has evolved tremendously. An invaluable contribution towards this gain in knowledge is attributable to advances in genomic research and technologies. Next-Generation Sequencing (NGS) approaches, as well as other techniques, have had a fundamen- tal impact on life sciences and many applied fields within our society. But the journey is far from over. Although well established, current NGS methods are unable to accurately resolve certain regions of the genome. This makes the detection of structural variants particularly challenging. Different cyto- genetic techniques have their own limitations as well. Even in concert, these methods cannot pro- vide a complete and high-resolution picture of the genome. This inability represents one of the last frontiers in genomics.
Conventional DNA Extraction Short-Read Sequencing Data 150 bp / Molecule
UHMW DNA Extraction * Optical Genome Mapping Data ≥ 230,000 bp / Molecule
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Throughout this brochure, protected product names may be used without being specifically marked as such.
*The DNA isolated from the Hamilton Long String STAR V contains Mbp length molecules which overall exceed the OGM quality threshold of 230 kbp post extraction, labeling, linearization, and imaging.
Together is Better
As experts in precision automated liquid handling, Hamilton has partnered with Bionano to automate the UHMW DNA extraction and labeling processes. Together, we empower the scientific com- munity by accelerating its ability to process a high number of samples in a reliable and reproducible man- ner. Our aim is to always provide the best possible solution, to elevate our customers.
See Sharper
Extract and Label Longer DNA Molecules Easier Than Ever Before
Although structural variants make a significant contribu- tion to human disease, such aberrations are frequently missed by NGS technology. The analysis of UHMW DNA by OGM provides a more comprehensive way of detecting such aberrations in the range of 500 bp up to megabase pairs in length. Structural variation discov- ery with OGM outperforms what can be achieved by sequencing-based workflows.
The manual preparation of labeled UHMW DNA samples can be challenging, due to sample viscosity and sensitivity to shearing. Manual protocols require users to be highly trained to achieve optimal results with regard to both quantity and quality. These factors also limit the number of samples that can be reasonably processed at a single time. Long String STAR V optimizes the laboratory workflow for purification and staining of ultra-long DNA molecules, increasing confidence about your sample quantity and quality. It drives consistency, as samples are always prepared with identical parameters, ensuring great yields and excellent quality. Adopt a full walk-away workflow for UHMW DNA extraction and labeling with increased sample throughput to take your sample preparation process to the next level. Replace manual preparations with consistent, auto- mated protocols Consistently obtain UHMW DNA molecules and su- preme labeling metrics Standardize and trace your sample workflow Reduce hands-on time for improved lab efficiency Process up to 12 samples per run and 24 samples in a single workday for extraction and cunduct labeling of up to 48 samples in 4h
Up to 99% structural variant detection
10,000X resolution compared to karyotype Routinely detect mosaic variants down to as little as 5% variant allele fraction To capitalize on these benefits, UHMW DNA (typically between 250 kbp up to > 1 Mpb in length) must be isolated from samples of interest. Instead of magnetic beads or silica spin columns, the procedure uses magnetic disks, which are covered by a nanostructured silica surface. This enables a high DNA binding capacity and helps to protect the bound DNA from shearing forces. In a second step, fluorescent labels are attached to a 6 bp sequence motif, on average occuring 20 times per 100 kbp in the human genome. This results in a uniquely identifiable genome-specific labeling pattern, allowing high resolution structural variant detection.
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The Tool for Novel Discoveries
IN FULL CONTROL OF EVERY STEP FOR MORE CONFIDENCE
Hamilton MagRods
Hamilton MagRods are specifically designed for optimal magnetic disk handling. MagRods can be operated with 5 mL CO-RE pipetting channels and therefore offer a broad range of flexibility. Handle magnetic disks anywhere on-deck Adjustable settings for both speed and range of movement Tunable to cater to sample type or workflow require- ments Freedom to optimize even single steps
The Long String STAR V is an Assay Ready Workstation with a standardized hardware configuration for automated magnetic disk handling. An optional hardware kit expands the instrument capabilities for enzymatic labeling steps. The product is suitable for Research Use Only (RUO). It is based on our latest application notes and greatest Microlab STAR V platform and features state- of-the-art CO-RE pipetting technology, as well as the Hamilton Quad CO-RE Gripper for plate transport steps. Our customers benefit from a turnkey solution that minimizes implementation times. Qualified Methods are being developed, together with our partners, and distributed along with the instrument. More than that, we value the strong support for our clients which serves well beyond initial installation.
EVERYTHING YOU NEED, WHENEVER YOU NEED IT
Hamilton MagRod Sleeves
Hamilton MagRod Sleeves are manufactured with the highest precision and are shaped for the best possible magnetic disk positioning. The perfect fit protects the MagRods from surrounding liquids and reagents.
For additional information about the Long String STAR V, including an explanatory animation video and an application notes with biological testing results, please access our webpage via the QR Code below.
Hamilton Elution Strips
Hamilton Elution Strips are used to separate the magnetic disks from the final UHMW DNA sample by centrifugation. The disk remains in the strip, while the eluate is drawn into a microtiter plate.
All necessary consumables are available directly from Hamilton and can be purchased separately. Free of human DNA, DNase, and RNase Sterile, in compliance with ISO 11135 Packaging size matches throughput needs
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Click, Click, Go
SETTING A SOLID FOUNDATION FOR OPTICAL GENOME MAPPING
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SAMPLE PREPARATION
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UHMW DNA EXTRACTION
Lysis and Digestion
DNA Binding to Disks
Disk Transfer to Elution Plate
Final Elution by Centrifugation
Disk Washing
We have designed the operator interface and programming styles with user friendliness in mind for faster familiarization. Intuitive editors offer simple ways to set up and run your method, with predefined parameters optimized by a team of Scientists from both Bionano and Hamilton. At the same time, you have full control over all method aspects.
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DIRECT LABEL AND STAIN (DLS)
DLE-1 Labeling
Proteinase K Digestion
DL-Green Clean-up
Homo- genization
DNA Staining
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Easily viewable color-coded lights indicate progress Customizable worklist templates for quick editing Run simulations for method optimization Loading dialogs specify required consumables and reagents Helper tools for new workflows and separate test methods Selected highlights include:
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More time to focus on steps ahead
Off-Deck Process
DATA COLLECTION ON SAPHYR SYSTEM
The established protocol is divided into simple lyse, bind, wash, and elution procedures that can be performed using kits from Bionano. Herein, genomic DNA is bound to, purified on, and released from a paramagnetic disk. Frozen cell pellets are manually resuspended and provided to the system (automated resuspension of cell pellets supported by the method). Subsequent steps are per- formed entirely on the Long String STAR V instrument. For final elution, the disks are transferred to strips pre-filled by the instrument with elution buffer, standing on top of a microtiter plate. Via a short off-deck centrifugation step, the final UHMW DNA samples are collected in the plate wells. After manual quality control and dilution, UHMW DNA samples can be provided back to the system for a fully automated labeling process. Only a final off-deck incuba- tion step is needed before samples can be loaded for data collection on the Saphyr ® and Stratys ® Systems.
Long String STAR V Process
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Optical Genome Mapping Process
ASSEMBLY, CROSS-
MAPPING AND STRUCTURAL VARIANT ANNOTATION
For products and services specific to data collection and analysis, please contact Bionano directly
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Specifications
Consumables
Physical Dimensions
Operating Conditions
Width
149 cm
Temperature Range
15 – 35°C
Depth
95.9 cm
Relative Humidity
15% – 85% with no condensation
Height with closed door
96.3 cm
Altitude
up to 2000 m above sea level
THE BASIS OF EVERY WORKFLOW
Height with open door
100.5 cm
Electrical Power Requirements
110 – 230V AC 50/60Hz +-5%
Weight
approx. 250 kg
1000 µL Pipetting Channels Technologies
1000 µL Pipetting Channel Specifications
We’re proud of the fact that we produce our own tips at various locations throughout Switzerland and the US. This is the only way we can meet our own high-quality standards; and not only ours, but also the most important ISO standards*.
Tip Size
Volume
Accuracy (%)
Precision CV (%)
CO-RE ® Technology
Independent Channels
50 µL
1 µL
5%
4%
capacitive Liquid Level Detection (cLLD)
300 µL
50 µL
2%
0.75%
Tip Type Detection
1000 µL
100 µL
2%
0.75%
Our goal is to provide the best possible components for our systems with our tips, because they’re the essence of every workflow. Nothing works without the appropriate consumables. That's why we offer an entire range of high-quality consumables that provide more security, higher efficiency, and thus more convenience. We also develop and produce smart consumables, which enable innovative solutions in new applications and research fields.
Order Configurations*
Consumables*
Description
Description
Manufacturer
Long String STAR V (extraction only)
50 µL Filtered Tips
Hamilton
300 µL Filtered Tips
Hamilton
1000 µL Filtered Tips
Hamilton
Add-On Option*
3.2 mm wide bore 1000 µL Filter Tip
Hamilton
Description Long String Labeling HW Kit (enabeling extraction
MagRod Sleeves
Hamilton
and labeling in one system) Long String STAR V Bench Swing Arm for Monitor (only available in combination with Bench)
Elution Strips
Hamilton
KingFisher 96 deep-well plate, v-bottom, poly- propylene
Thermo Fisher Scientific
20 mL Reagent Reservoirs
Hamilton
Hamilton consumables are produced in an ISO Class 8 Clean Room, based on ISO 14644 standards, as well as ISO 9001 and ISO 13485. Hamilton goes beyond relying on just statistical analyses of quality to assure the highest product quality (e.g. each CO-RE tip is checked for concentricity of its orifice and the absence of flash from the molding process).
Microplate 96/U, Wells Clear, RecoverMax
Eppendorf
*For ordering details, please get in touch with your local Hamilton sales representative.
2.0 mL Screw Cap Microtubes
Sarstedt
PCR Comfrot Lids
Hamilton
MultiScreen HTS 96 Well-Filter Plate with MCE-Membrane
Merk
96 well PCR Plate
Hamilton
0.5 mL SCrew Cap Microtubes
Sarstedt
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Long String Genomics
New Frontiers
OPTICAL GENOME MAPPING - THE FUTURE IS NOW
MOVING FORWARD BY CLOSING GAPS
Use UHMW DNA to perform OGM for unbiased, genome-wide detection of structural variants across a broad range of research applications:
An alternative to traditional cytogenetic workflows to detect more chromosomal aberrations in a sin- gle assay OGM detects the structural variants that are missed by sequencing with higher resolution than traditional cytogenetic methods. Reset the benchmark for detecting chromosomal aberrations.
Using the Long String STAR V in your laboratory will provide you with the opportunity to extract and label UHMW DNA easier than ever before with sample throughput surpassing manual capabilities. Get the power to bring your workflow performance to the next level. Human handling errors are a thing of the past, and the user-to-user variation seen with manual protocols is replaced by automated consistency and reproducibility. The Long String STAR V from Hamilton and OGM solutions from Bionano add another layer of discovery to your genetic experiments. With the ability to reliably extract and label ultra-long DNA molecules paired with the Bionano Saphyr system, all classes of structural variants are revealed and short-read sequencing blindspots can be unveiled. Together, Long String STAR V and OGM deliver a view that empowers you to overcome challenges and discover more.
Constitutional genetic disorders
Hematologic malignancies
Solid tumors
Cell bioprocessing quality control Gene discovery and therapy Reference genome assembly
Complement data across the variant continuum by combining OGM with NGS-based methods
By combining OGM and NGS, you can characterize both structural and single nucleotide variants, providing a comprehensive analysis of genomic variation that can impact human health. Reveal more that matters for powerful genetic discovery.
You have just turned from page 3 to page 12 and the OGM Data / molecule has covered all pages, while conventional NGS Data has taken only a very small space on page 3.
For more information about potential applications for Optical Genome Mapping with UHMW DNA, visit bionanogenomics.com.
* The DNA isolated from the Hamilton Long String STAR V contains Mbp length molecules which overall exceed the OGM quality threshold of 230 kbp post-extraction, labeling, linearization, and imaging.
Yes, that long!
UHMW DNA Extraction * Optical Genome Mapping Data ≥ 230,000 bp / Molecule
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Automating your Imagination
We drive innovation to improve people's lives
HAMILTON ROBOTICS
We develop and produce state-of-the-art Liquid Handling Systems and laboratory automation technologies. Devote yourself to new ideas, trans- forming your ideas into reality. The entire product portfolio is characterized by maximum reliability, maximum efficiency, flexibility and user-friendliness. From production to service and support, the focus is always on your individual needs.
HAMILTON
Hamilton has stood for Life Sciences and Medical Technology since 1966. A visionary idea developed into a strong global presence. Our spirit has always remained the same: To research, develop and produce with passion, courage and curios- ity. The desire to discover new technological solutions, innovations, and benefit humanity is our mission. We strive to set our goals high and continue to meet the measure of excellence.
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To find a subsidiary or distributor in your area, please visit, www.hamiltoncompany.com/support.
United States +1-775-858-3000 United Kingdom, Ireland +44 121 272 92 80 Brazil +55 11 95914 5000 China +86 21 6164 6567
Denmark, Norway, Sweden, Finland +46 8410 27 373 France +33 184 008 420 Germany, Austria, Switzerland +49 89 248 804 804
Benelux +31 40 209 178 0 Italy +39 039 930 06 06 Japan +81 3 6435 6850 Spain, Portugal +34 930 186 262
Web: www.hamiltoncompany.com/robotics Email: infoservice@hamiltonrobotics.com
© 2022 Hamilton Company. All rights reserved. All trademarks are owned and/or registered by Hamilton Company in the U.S. and/or other countries. Lit. No. B-2205-02— 01/2024 Bionano , Saphyr and Stratys are trademarks of Bionano, Inc.
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