Together is Better
As experts in precision automated liquid handling, Hamilton has partnered with Bionano to automate the UHMW DNA extraction and labeling processes. Together, we empower the scientific com- munity by accelerating its ability to process a high number of samples in a reliable and reproducible man- ner. Our aim is to always provide the best possible solution, to elevate our customers.
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Extract and Label Longer DNA Molecules Easier Than Ever Before
Although structural variants make a significant contribu- tion to human disease, such aberrations are frequently missed by NGS technology. The analysis of UHMW DNA by OGM provides a more comprehensive way of detecting such aberrations in the range of 500 bp up to megabase pairs in length. Structural variation discov- ery with OGM outperforms what can be achieved by sequencing-based workflows.
The manual preparation of labeled UHMW DNA samples can be challenging, due to sample viscosity and sensitivity to shearing. Manual protocols require users to be highly trained to achieve optimal results with regard to both quantity and quality. These factors also limit the number of samples that can be reasonably processed at a single time. Long String STAR V optimizes the laboratory workflow for purification and staining of ultra-long DNA molecules, increasing confidence about your sample quantity and quality. It drives consistency, as samples are always prepared with identical parameters, ensuring great yields and excellent quality. Adopt a full walk-away workflow for UHMW DNA extraction and labeling with increased sample throughput to take your sample preparation process to the next level. Replace manual preparations with consistent, auto- mated protocols Consistently obtain UHMW DNA molecules and su- preme labeling metrics Standardize and trace your sample workflow Reduce hands-on time for improved lab efficiency Process up to 12 samples per run and 24 samples in a single workday for extraction and cunduct labeling of up to 48 samples in 4h
Up to 99% structural variant detection
10,000X resolution compared to karyotype Routinely detect mosaic variants down to as little as 5% variant allele fraction To capitalize on these benefits, UHMW DNA (typically between 250 kbp up to > 1 Mpb in length) must be isolated from samples of interest. Instead of magnetic beads or silica spin columns, the procedure uses magnetic disks, which are covered by a nanostructured silica surface. This enables a high DNA binding capacity and helps to protect the bound DNA from shearing forces. In a second step, fluorescent labels are attached to a 6 bp sequence motif, on average occuring 20 times per 100 kbp in the human genome. This results in a uniquely identifiable genome-specific labeling pattern, allowing high resolution structural variant detection.
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